• 1
  • 1

Applications of PCR detection


If you are looking for high-quality products, please feel free to contact us and send an inquiry, email:

What is PCR testing?

Polymerase chain reaction (PCR) uses DNA fragments as templates, with the participation of DNA polymerase and nucleotide substrates, to amplify DNA to a sufficient amount for structural and functional analysis. PCR detection method is of great significance for the rapid diagnosis of clinical bacterial infectious diseases.

What is the principle of PCR?

The principle of PCR is to amplify DNA fragments located between two known sequences, similar to the natural DNA replication process. Taking the DNA molecule to be amplified as a template, and a pair of oligonucleotide fragments complementary to the 5' end and 3' end of the template as primers, under the action of DNA polymerase, according to the semi-reserved replication mechanism of the template, the chain is separated. Extend until new DNA synthesis is complete and repeat the process to amplify the target DNA fragment.

Applications of PCR detection

Applications of PCR detection:

1. Pathogen detection

Currently, fluorescent quantitative PCR detection technology can detect gonococcus, Chlamydia trachomatis, Ureaplasma urealyticum, human papilloma virus, herpes simplex virus, human immunodeficiency virus, hepatitis virus, influenza virus, Mycobacterium tuberculosis, eb virus and cytomegalo Quantitative determination of pathogens such as viruses. Compared with traditional detection methods, it has the advantages of high sensitivity, less sampling, fast and convenient.

2. Prenatal diagnosis

So far, there is no treatment for genetic diseases caused by changes in genetic material. Only prenatal monitoring can reduce the birth of sick babies and prevent the occurrence of various genetic diseases. For example, reducing the number of children with an X-linked genetic disorder is a non-invasive approach that is easily accepted by pregnant women.

3. Evaluation of drug efficacy

Quantitative analysis of hepatitis B virus (HBV) and hepatitis C virus (HCV) has shown that the amount of virus correlates with the efficacy of certain drugs. High levels of HCV expression are insensitive to the effects of interferon, whereas low titers of HCV are sensitive to the effects of interferon; serum levels of HBV-DNA have decreased briefly during lamivudine treatment, and if increased again or more than before level, indicating that the virus has mutated. Such as the application and significance of PCR technology in HBV detection:

(1) Know the amount of hepatitis B virus in the body.

(2) Whether to copy.

(3) Whether it is contagious, and how contagious it is.

(4) Whether it is necessary to take medicine.

(5) Whether abnormal liver function is caused by virus.

(6) Determine which antiviral drug is suitable for the patient.

(7) Determine the efficacy of drug treatment.

4. Tumor gene detection

Although tumor pathogenesis remains unclear, it is widely accepted that mutations in related genes are potential causes of oncogenic transformation. In many tumors, increased expression and mutations of oncogenes can appear at an early stage. Real-time quantitative PCR can not only effectively detect gene mutations, but also accurately detect the expression of oncogenes. At present, this method has been used to detect the expression of telomerase hTERT gene, chronic myeloid leukemia WT1 gene, tumor ER gene, prostate cancer PSM gene, tumor-associated virus gene and other genes. With the continuous discovery of new tumor-related genes, real-time PCR technology will play a greater role in tumor research.

Professional manufacturer of Nucleic Acid (DNA & RNA) Extraction and Analysis products supplier

GENETURE is a group company,we own two factories: Ascend and Dianrun,to provide one stop solution of Nucleic Acid Extraction and Analysis,including solution for COVID-19. Geneture provides high quality and professional Nucleic Acid Extraction Reagents, Lab consumables, Real-time PCR consumables and test machines. 

GENETURE main products including: Nucleic acid extraction or purification kit,Automatic nucleic acid extractor, PCR system, PCR kit, Magnetic beads, and lab consumables of 96 well deep plate,Magnetic rod comb,PCR tube,PCR plate,Pipette tips,centrifuge tubes. If you have any questions about us or Nucleic Acid (DNA & RNA) Extraction and Analysis products, feel free to contact us.


Mobile: +86 150 1002 8687

Inquiry us

High Purity Germanium Sulfide GeS2 Powder CAS 12025-34-2, 99.99%

High Purity Molybdenum Boride MoB2 Powder CAS 12006-99-4, 99%

Metal Alloy 8.92g/Cm3 High Purity Polished Copper Plate

Metal Alloy High Density Tungsten Alloy Rod Grind Surface Tungsten Alloy Bar

Metal Alloy 18.5g/cm3 Polished Tungsten Heavy Alloy Plate

Metal Alloy 18g/cm3 High Density Tungsten Alloy Ball

High Purity 3D Printing Nickel Alloy IN718 Powder

High Purity 3D Printing Alloy CoCrW Powder

High Purity Chromium Diboride CrB2 Powder CAS 12007-16-8, 99%

High Purity Nano Ag Silver powder cas 7440-22-4, 99%

High Purity Calcium Nitride Ca3N2 Powder CAS 12013-82-0, 99.5%

High Purity Nano Hafnium Hf powder CAS 7440-58-6, 99%

High Purity Titanium Sulfide TiS2 Powder CAS 2039-13-3, 99.99%

High Purity Tungsten Silicide WSi2 Powder CAS 12039-88-2, 99%

High Purity 3D Printing Powder 15-5 Stainless Steel Powder

High Purity Zirconium Nitride ZrN Powder CAS 25658-42-8, 99.5%

High Purity Silicon Sulfide SiS2 Powder CAS 13759-10-9, 99.99%

High Purity Colloidal Silver Nano Silver Solution CAS 7440-22-4

Supply Magnesium Granules Mg Granules 99.95%

High Purity 3D Printing 304 Stainless Steel Powder

Our Latest Products

High Purity Germanium Sulfide GeS2 Powder CAS 12025-34-2, 99.99%

TRUNNANO is a reliable supplier for high purity Germanium Sulfide GeS2 Powder CAS 12025-34-2, 99.99%.…

High Purity Molybdenum Boride MoB2 Powder CAS 12006-99-4, 99%

TRUNNANO is a reliable supplier for high purity Molybdenum Boride MoB2 Powder CAS 12006-99-4, 99%.…

Metal Alloy 8.92g/Cm3 High Purity Polished Copper Plate

TRUNNANO is a reliable supplier of Metal Alloy 8.92g/Cm3 High Purity Polished Copper Plate.…